HRP Anti-beta III Tubulin antibody [2G10] (ab196638) (2024)

Overview

• Product name

  HRP Anti-beta III Tubulin antibody [2G10]
  See all beta III Tubulin primary antibodies

• Description

  HRP Mouse monoclonal [2G10] to beta III Tubulin

• Host species

  Mouse

• Conjugation

  HRP

• Tested applications

  Suitable for: IHC-P, WBmore details

• Species reactivity

  Reacts with: Mouse, Rat, Human
  Predicted to work with: Cow, Common marmoset

• Immunogen

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• Positive control

  • WB: Human, mouse and rat brain tissue lysates.IHC: Human cerebellum tissue.

• General notes

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

• Storage buffer

  pH: 7.40
  Preservative: 0.1% Proclin 300 Solution
  Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS

• Concentration information loading...

• Purity

  Affinity purified

• Clonality

  Monoclonal

• Clone number

  2G10

• Isotype

  IgG2a

• Research areas

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microtubules
  • Tubulin

  • Neuroscience
  • Development

Associated products

• Alternative Versions

  • Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] (ab195879)
  • Alexa Fluor® 647 Anti-beta III Tubulin antibody [2G10] (ab195880)
  • Anti-beta III Tubulin antibody [2G10] - BSA and Azide free (ab264097)
  • Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)

• Recombinant Protein

  • Recombinant Human beta III Tubulin protein (ab140581)

• Related Buffer

  • ECL Substrate Kit (High Sensitivity) (ab133406)

Applications

Target

• Function

  Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

• Tissue specificity

  Expression is primarily restricted to central and peripheral nervous system.

• Involvement in disease

  Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.

• Sequence similarities

  Belongs to the tubulin family.

• Domain

  The highly acidic C-terminal region may bind cations such as calcium.

• Post-translational
  modifications

  Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

• Cellular localization

  Cytoplasm > cytoskeleton.

• Information by UniProt

• Database links

  • Entrez Gene: 768070 Cow
  • Entrez Gene: 10381 Human
  • Entrez Gene: 22152 Mouse
  • Entrez Gene: 246118 Rat
  • Omim: 602661 Human
  • SwissProt: Q2T9S0 Cow
  • SwissProt: Q13509 Human
  • SwissProt: Q9ERD7 Mouse

  see all

• Alternative names

  • beta 3 tubulin antibody
  • beta 4 antibody
  • beta-4 antibody

  see all

Images

Protocols

Datasheets and documents

References (0)